Centrifugation

Zvemukati

• Mhando dze centrifugation
• Mienzaniso ye centrifugation

Iyo centrifugation Iyo nzira yekuparadzanisa zvinhu zvine simba kubva kune zvinwiwa zvehuwandu hwakasiyana mumusanganiswa, sekureba sekutanga zvisina kunyungudika, uchishandisa rotary simba kana centrifugal simba.

Kune izvi, chiridzwa chinonzi centrifuge kana centrifuge chinowanzo shandiswa, chinotenderera musanganiswa pane yakagadziriswa uye yakatemwa axis.

Seizvo zita rayo rinoratidza (centrifuge: kutiza kubva pakati), iri simba rinowanzo sundira zvinhu zvakanyanyisa kubva kunze kweiyo kutenderera, zvichisiya zvisina kuomarara pakati chaipo. Izvo zvinopesana necentripetal simba.

• Onawo: Chromatography

Mhando dze centrifugation

• Kusiyanisa. Zvichienderana nemusiyano wehuwandu hwezvinhu, ndiyo yekutanga asi isina maitiro.
• Isopychnic. Unyanzvi uhu hunoshandiswa, semuenzaniso, kupatsanura zvidimbu zvehukuru hwakaenzana asi nehukuru hwakasiyana.
• Zonal. Musiyano weiyo sedimentation mwero wezvinhu (nekuda kwemazhinji akasiyana) anoshandiswa kuvapatsanura mune yakapihwa nguva yekukwirisa.
• Ultracentrifugation. Simba rayo rinotendera kupatsanurwa kwemamorekuru uye subcellular zvinhu.

Mienzaniso ye centrifugation

1. Muchina wekuwachisa. Chishandiso ichi chinoshandisa simba re centrifugal kupatsanura mbatya (solid) kubva mumvura (liquid) zvichibva pakudenga kwavo. Ndokusaka mbatya dzinowanzoita seyakaoma kana dzabviswa mukati.
2. Maindasitiri emukaka. Mukaka uri centrifuged kupatsanura mvura uye lipid zvemukati, sezvo iyo yekupedzisira inoshandiswa kugadzira bata, kana mukaka wakaomeswa kubva kune yasara.
3. Motokari munyonga. Kana tichifambisa tichimhanya kuburikidza nekakona mumugwagwa, isu tinowanzo kunzwa simba richitiburitsa kunze kwemugwagwa, kure neiyo axis yekukombama. Ndiro simba re centrifugal.
4. Kuwana enzymes. Muindasitiri yezvokurapa neyemishonga, centrifugation inowanzo shandiswa kuwana mamwe maenzymes kubva kune akakosha maseru anovaburitsa.
5. Kuparadzaniswa kweDNA. Isopycnic centrifugation inowanzo shandiswa mumarabhoritari emarabhoritari kupatsanura maseru eDNA uye kubvumidza kuenderera mberi nekufunda uye kugadzirisa.
6. Chikafu cheecliacs. Kana zvasvika pakupatsanura protein kubva ku gluten kubva kuzvikafu zvine iyo, iyo centrifugation maitiro akakosha. Inoitwa pane starch paste, ine zvemukati zvegluten zvinosvika 8%, uye zvinoderedzwa kusvika pazasi pe2% mumashure akateedzana.
7. Kuongororwa kweropa Centrifuge inoshandiswa kupatsanura zvinhu zveropa, zvakaita seplasma nezvimwe zvinhu zvinowanzo sanganiswa mukati maro.
8. Kumhanyisa kwenzvimbo. Mune akasiyana maindasitiri ezvekudya, senge kubheka kana zviyo, centrifugation inomhanyisa iyo sedimentation maitiro ayo anongoerekana aunza giravhiti, ichideredza nguva yekumirira yezvinhu zvakasvibirira.
9. Kuchenesa iro latex. Mune iyo latex indasitiri, zvinodikanwa kuchenesa chinhu, chine pamusoro pacho chinonyanya kutevedzera kunamatira kwezvimwe zvidimbu, uye izvi zvinoitwa kuburikidza necentrifugation, zvichipiwa yakaderera density yeiyo chinhu.
10. Kuomesa kwemarara. Kumwe kushandiswa kwemaindasitiri kweiyo centrifuge ndiko kuomeswa kwemakristasi kana zvimwe zvinhu zvine kugadzirwa kunoenderana nemvura. Sezvo inotenderera, mvura inopatsanuka kubva kune yakasviba uye inoraswa, ichisiya iyo inodiwa yakasimba isina mvura.
11. Kuchenesa tsvina. Iko centrifugation yemvura yakasviba inobvumira kuburitswa kwezvinhu zvakawandisa mukati, kwete chete zvakasimba, asi kunyange maoiri, mafuta nezvimwe zvinhu zvisingadiwe izvo, kana zvangoiswa centrifuged, zvinogona kuraswa.
12. Mapaki evaraidzo. Vatasvi vazhinji vepaki yekuvaraidza vanoshandisa simba re centrifugal kuti vagadzire vacuum mhedzisiro kune ivo vatasvi, avo vanokurumidza kutenderedzwa pane yakatarwa axis, zvakasungwa zvakasungirirwa pachigaro chinovatadzisa kukandwa kunze kweiyo axis yekutenderera.
13. Pirouette midhudhudhu. Iye mudhudhudhu mune imwe nharaunda ndeye yakasarudzika yemasikisi, anokwanisa kutyaira padenga repadenga achishora giravhiti. Izvi zvinokwanisika kuzviita mushure mekuita kutendeuka kwakawanda pane imwechete yakatwasuka axis, kuunganidza kumhanya uye kuzviisa pasi pesimba re centrifugal rinoomerera kune iro remukati medanho. Pakupedzisira iri simba rinenge rakakura zvekuti richakwanisa kuenzanisa kufamba uye kushora giravhiti.
14. Kufarira kwenzira dzechitima. Kurwisa simba re centrifugal, matirakiti ezvitima anowanzo tenderedzwa mumakomba, achishandisa kuramba kuitira kuti irege kukundwa nesimba rinorisundira kunze uye risingakundike.
15. Shanduro yenyika. Icho chikonzero chekuti simba rekunonoka reZuva haritisundidzire takananga mairi zvakare nekuda kwesimba re centrifugal iro, kana richitenderera pane axis yamambo wezuva, rinorisundira kunze, richirwisa uye kuenzanisa kukwezva kunokwezva.

Dzimwe nzira dzekuparadzanisa masanganiswa

• Kuchenesa
• Distillation
• Chromatography
• Kugumisa
• Magnetization